- Apo-A1 is a 29.0 kDa protein produced in the liver and intestine, and secreted as the predominant constituent of nascent high-density lipoprotein (HDL) particle. Apo-A1, which is found exclusively in HDL, has a unique ability to capture and solubilize free cholesterol. This Apo- 1 ability enables HDL to remove excess peripheral cholesterol and return it to the liver for recycling and excretion. This process, called reverse cholesterol transport, is though to inhibit atherogenesis. For this reason HDL is also known as the "good cholesterol." The therapeutic potential of Apo-A1 has been recently assessed in patients with acute coronary syndromes, using a recombinant form of a naturally occurring variant of Apo-A1 (called Apo-A1 Milano). The availability of recombinant normal Apo-A1 should facilitate further investigation into the potential usefulness of apoA-I in preventing atherosclerotic vascular diseases. Recombinant human Apo-A1 is a 28.2 kDa protein of 244 amino acid residues.
- Product Overview
- Human APOA1 expressed in E.coli
- CAT# CSC-CTK0093-100 (100 μg); CAT# CSC-CTK0093-500 (500 μg)
- Greater than 97% as determined by SDS-PAGE and HPLC analysis.
- Endotoxin Level
- Less than 1 EU/μg.
- Lyophilized from a sterile-filtered solution with no additives.
- Please centrifuge product before opening vial. The lyophilized protein should be reconstituted with distilled, sterile water to a concentration of 0.1-1.0 mg/ml. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
- Storage & Stability
- The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.
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