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ActoFactor™ Recombinant Human Allograft inflammatory factor 1

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0322
  • Description
  • AIF-1 is expressed in macrophages and neutrophils. The expression of AIF-1 transcripts is upregulated by IFN-g in rat macrophages. AIF-1 is expressed selectively in human macrophage- like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. In quiescent cultured human vascular smooth muscle cells synthesis of AIF-1 is induced by IFN-g, IL-1b, and conditioned medium of T-cells. Overexpression of AIF-1 in human VSMCs results in enhanced growth of these cells. AIF-1 is expressed during apoptosis rat mammary gland and ventral prostate tissues. Allograft Inflammatory Factor 1 is expressed by several tumor-associated activated macrophages and microglial cells in rat and human gliomas. There is an evident relationship of AIF1-expressing activated macrophages and microglial cells with tumor malignancy in humans. Human AIF-1 protein shares 98% homology/identity with that of rat. Recombinant human AIF-1 contains a total of 155 amino acids and has a molecular Mass of 17.7 kDa. The human AIF-1 is fused to a 9 amino acid long N-terminal His tag.
  • Species
  • Human
  • Product Overview
  • Human AIF1 expressed in E.coli
  • Molecular Mass
  • 17.7 kDa
  • Size
  • CAT# CSC-CTK0322-10 (10 μg); CAT# CSC-CTK0322-50 (50 μg)
  • Expression System
  • E.coli
  • Purity
  • Greater than 90% as determined by SDS-PAGE analysis.
  • Activity
  • N/A
  • Endotoxin Level
  • Less than 1 EU/μg.
  • Formulation
  • Sterile filtered and lyophilized from a 0.5 mg/ml p rotein solution in 20 mM Tris buffer and 50 mM NaCl (pH 7.5).
  • Reconstitution
  • Please centrifuge product briefly before opening the vial. The lyophilized rHu AIF-1 should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be further diluted into other aqueous buffers and stored at -20°C for future use.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted rHu AIF-1 should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, we recommend the addition of a carrier protein (0.1% HSA or BSA). Avoid repeated freeze / thaw cycles.

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      45-1 Ramsey Road, Shirley, NY 11967, USA
      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
      Europe
      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com