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ActoFactor™ Recombinant Human Activin A receptor, type I (Plant)

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0115
  • Description
  • Activins are homodimers or heterodimers of the various β subunit isoforms, belonging to the TGF-beta family. Mature Activin B has two chains of 123 amino acid residues (betaB-betaB). Activin exhibits a wide range of biological activities, including mesoderm induction, neural cell differentiation, bone remodelling, haematopoiesis, and reproductive physiology. Activins plays a key role in the production and regulation of hormones such as FSH, LH, GnRH and ACTH. Inhibins/Activins are proteins that are formed by the dimerization of two subunits, i. e. an α (alpha) with either beta A (βA) – Inhibin A or beta B (βB) – Inhibin B. The subunits betaA and betaB can also form homodimers or heterodimers called Activin: Activin A (betaA -betaA), Activin B (betaB -betaB) and Activin AB (betaA -betaB). The Activin gene family comprises the additional, but poorly characterized members' Activin betaC (βC), beta D (βD) and beta E (βE). As with other members of the super-family, Activins interact with two types of cell surface trans- membrane receptors (Types I and II) which have intrinsic serine/threonine kinase activities in their cytoplasmic domains, Activin type 1 receptors, ACVR1, ACVR1B, ACVR1C and Activin type 2 receptors. Recombinant human Activin B is a polypeptide chain containing 123 amino acids (293-406; P09529INHBB_HUMAN) and a His-tag at the N-terminal end. It has a predicted molecular mass of 14 kDa. Human recombinant Activin B expressed in Nicotiana benthamiana is produced by transient expression in non-transgenic plants and is purified by sequential chromatography (FPLC). This product does not contain any animal–derived components or impurities.
  • Species
  • Human
  • Product Overview
  • Human ACVR1 expressed in Nicotiana benthamiana
  • Molecular Mass
  • 14 kDa
  • Size
  • CAT# CSC-CTK0115-10 (10 μg); CAT# CSC-CTK0115-50 (50 μg)
  • Expression System
  • Nicotiana benthamiana
  • Purity
  • Greater than 97% as determined by SDS-PAGE analysis.
  • Activity
  • The biological activity of Activin B is measured by its ability to inhibit mouse plasmacytoma cell line (MPC-11) cells proliferation. An ED50 <5 ng/ml is required to stimulate a half-maximal response at cytokine saturation. Note: Since applications vary, each investigator should titrate the reagent to obtain optimal results.
  • Endotoxin Level
  • Less than 0.04 EU/μg.
  • Formulation
  • Lyophilized from a concentrated, sterile protein solution in 0.05 M Tris-HCl buffer (pH 7.4).
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 50 μg/ml. This solution can then be diluted into other aqueous buffers and stored at -20°C for future use. At higher concentrations the solubility may be reduced and multimers generated. Optimal concentration should be determined for specific application and cell lines.
  • Storage & Stability
  • The lyophilized protein, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted protein should be used immediately or stored long-term in undiluted working aliquots at -20°C. For long-term storage, it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid repeated freeze-thaw cycles.

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      Tel: 1-631-626-9181
      Fax: 1-631-614-7828
      Email: info@creative-bioarray.com
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com