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ActoFactor™ Human Angiostatin Kringles 1-4

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  • Specification
  • Recommended Products
  • Cat.No.
  • CSC-CTK0125
  • Description
  • There are several proteolytic fragments or specific domains of proteins that act as inhibitors of angiogenesis. These include fragments of plasminogen such as Angiostatin protein kringles 1-4 and kringles 1-5, Endostatin, Restin, PEX, the N-terminal fragment of prolactin, and the N- terminally truncated platelet factor. Angiostatin is a proteolytic protein fragment of plasminogen that is comprised of the first 4 kringle regions. Angiostatin is an angiogenesis inhibitor. Ang K1-4 prevents the growth of endothelial cells, and its systemic administration inhibits the growth of primary carcinomas in mice. Angiostatin Kringles 1-3 segment has a larger inhibitory activity than the Angiostatin Kringles 1-4 fragment. The protease-activated Angiostatin Kringles 1-5 is the most potent plasminogen fragment with over 50 times larger endothelial cell specific inhibitory activity. Angiostatin Kringles 1-5 systemic administration inhibits growth of fibrosarcoma and significantly reduces neovascularization. Angiostatin is a 38 kDa protein fragment of the plasminogen composed of the first 4 kringle domains of plasminogen. Angiostatin K1-4 is also named plasminogen kringles 1-4 and PK1-4. It is manufactured by the protelytic cleavage of plasminogen by a serine protease from several prostate carcinoma cell lines. The manufacturing of angiostatin by pancreatic cancer cells can be inhibited by TGF-beta 1 along with plasminogen activator inhibitor type-1 (PAI1).|Human Angiostatin K1-4, produced from human fluid, is a glycosylated polypeptide chain which migrates as a doublet at 50 kDa on SDS-PAGE analysis.
  • Species
  • Human
  • Product Overview
  • Plg from Human Fluid
  • Molecular Mass
  • 50 kDa
  • Size
  • CAT# CSC-CTK0125-10 (10 μg); CAT# CSC-CTK0125-50 (50 μg)
  • Expression System
  • Human Fluid
  • Purity
  • Greater than 98% as determined by SDS-PAGE.
  • Activity
  • Human Angiostatin Kringles 1-4 significantly inhibits basic-FGF induced endothelial cell proliferation and migration at concentrations ranging from 300 nM to 1.0 μM.
  • Endotoxin Level
  • N/A
  • Formulation
  • Lyophilized from a solution (1 mg/ml) containing 20 mM Hepes buffer (pH 8.2) and 20 mM NaCl.
  • Reconstitution
  • Please centrifuge product briefly before opening vial. The lyophilized protein should be reconstituted in sterile, ultra-pure water to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into water or other aqueous buffers and stored at -20°C to -70°C for future use. For further dilution, carrier protein (0.1% BSA or HSA) should be added to avoid loss of bioactivity.
  • Storage & Stability
  • The lyophilized powder, though stable at room temperature for up to 3 weeks, is best stored desiccated at -20°C. Reconstituted Ang K1-4 should be used immediately or stored long-term in undiluted working aliquots at -20°C. Avoid repeated freeze/thaw cycles.

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      Tel: 1-631-626-9181
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      Tel: 44-207-097-1828
      Email: info@creative-bioarray.com
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Contact Us
USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-097-1828
Email: info@creative-bioarray.com