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8-MG-BA

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  • Cat.No.
  • CSC-C0467
  • Description
  • Established from the frontal brain lobe of a 54-year-old woman with glioblastoma multiforme in 1989 (pre-treatment)
  • Recommended Medium
  • 80-85% MEM (with Earles salts) + 15-20% h.i. FBS
  • Organism
  • Human
  • Morphology
  • epithelial-like, adherent cells growing as monolayer
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hyperdiploid karyotype with 15% polyploidy - 52(47-52)<2n>X, -X, +1, +1, +3, +5, +6, +7, +8, -19, +2-4mar, add(1)(p34-36;hsr), add(1)(p34-36), der(1)t(1;17)(q11-12;q12-21), der(3;5)(q10;q10), i(5p), del(6)(q24-25), del(7)(p14), i(7p), i(8
  • Quality Control
  • Mycoplasma: contamination was eliminated with Ciprobay (ciprofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin (+), cytokeratin-7 -, cytokeratin-8 (+), cytokeratin-17 -, cytokeratin-18 -, cyto
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • frontal brain lobe

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45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com