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42-MG-BA

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  • Cat.No.
  • CSC-C0466
  • Description
  • Established from the temporal lobe brain biopsy from a 63-year-old man with glioblastoma multiforme in 1990 (prior to treatment)
  • Recommended Medium
  • 80% mixture of RPMI-1640 + MEM (with Earles salts) (at 1:1) + 20% h.i. FBS
  • Organism
  • Human
  • Morphology
  • flat epithelial-like polygonal cells growing adherently as monolayer or, if confluent, as multilayers with processes
  • Culture Properties
  • monolayer
  • Karyotype
  • Human flat-moded hypertetraploid karyotype with 8% polyploidy - 88-95<4n>XX, -Y, -Y, +4, -5, -5, -7, -8, -9, -9, +11, +11, -13, -13, +14, +14, +15, -16, -17, -17, -18, -18, -19, -21, -22, +14mar, add(1)(p1?), del(1)(q21.2), del(2)(q13)x2, dup(2)(p12p2?4)a
  • Quality Control
  • Mycoplasma: contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays
    Immunology: cytokeratin -, desmin -, endothel -, GFAP +, neurofilament -, vimentin +
    Viruses: ELISA: revers
  • Storage and Shipping
  • Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-2 x 10^6 cells/ampoule; ship in dry ice; store in liquid nitrogen
  • Tissue
  • glioma

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USA
45-1 Ramsey Road, Shirley, NY 11967, USA
Tel: 1-631-626-9181
Fax: 1-631-614-7828
Email: info@creative-bioarray.com

Europe
Tel: 44-207-048-3343
Email: info@creative-bioarray.com